Ber-H2 (CD30) immunohistochemical staining in malignant melanoma

Malignant melanoma can be included in the differential diagnosis of Hodgkin 's disease, anaplastic large cell lymphoma, or embryonal carcinoma These ma lignancies express CD30, a marker of diagnostic value. A retrospective immu nohistochemical study was undertaken to determine the frequency of immunore activity of Ber-IIB (anti-CD30 monoclonal antibody) in malignant melanoma A rchival paraffin-embedded tissue from 24 primary and metastatic lesions was used. No Ber-H2 labeling was observed in the majority of the studied cases . Variable weak cytoplasmic staining was present in only one case. The find ings are compared with the previous reports claiming frequent CD30 expressi on in malignant melanoma We discuss issues pertaining to the interpretation of the Ber-H2 MC staining in formalin-fixed, paraffin-embedded tissue.