Immunohistochemical detection of the lipid peroxidation product 4-hydroxynonenal after experimental brain injury in the rat

Citation
Dq. Zhang et al., Immunohistochemical detection of the lipid peroxidation product 4-hydroxynonenal after experimental brain injury in the rat, NEUROSCI L, 272(1), 1999, pp. 57-61
Citations number
27
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROSCIENCE LETTERS
ISSN journal
03043940 → ACNP
Volume
272
Issue
1
Year of publication
1999
Pages
57 - 61
Database
ISI
SICI code
0304-3940(19990903)272:1<57:IDOTLP>2.0.ZU;2-9
Abstract
This study examined the accumulation of the cytotoxic lipid peroxidation pr oduct 4-hydroxynonenal (HNE) after lateral fluid percussion (FP) brain inju ry in rats. A diffuse distribution of HNE-immunoreactivity (HNE-ir) was obs erved in the cortex and hippocampus of the ipsilateral, but not of the cont ralateral, hemisphere at 30 min, 6 h, 24 h, and 48 h after brain injury. Th e HNE-ir was well-localized in cell bodies of the ipsilateral cortex and th e CA3 pyramidal layer in the ipsilateral hippocampus. Because HNE's interac tion with certain proteins causes protein dysfunction and HNE, in vitro, ca uses neuronal cell damage, the present results suggest that HNE's interacti on with neuronal proteins may contribute to neuronal damage in the ipsilate ral cortex and hippocampus after brain injury. (C) 1999 Elsevier Science Ir eland Ltd. All rights reserved.