Objectives-Despite observation of adverse reproductive effects of toluene,
including alterations of serum gonadotropins (luteinising hormone (LH) and
follicle stimulating hormone (FSH)) in humans, Little is known of the mecha
nism of toxicity. The hypothesis was tested that toluene acutely suppresses
pulsatile gonadotropin secretion by measuring LH and FSH at frequent inter
vals during controlled exposure to toluene.
Methods-Women in the follicular and luteal phases of the menstrual cycle an
d men were randomised to inhale filtered air with or without 50 ppm toluene
through a mouthpiece for 3 hours (19% of the OSHA permissable exposure Lim
it). Blood was sampled by intravenous catheter at 20 minute intervals for 3
hours before, 3 hours during, and 3 hours after exposure. Plasma LH, FSH,
and testosterone were measured. Pulse amplitude, pulse frequency, and mean
concentrations of LH and FSH for each of the 3 hour periods before, during
and after exposure to toluene versus sham exposure were calculated with the
ULTRA pulse detection program and compared by analysis of variance (ANOVA)
with repeated measures.
Results-In men mean concentrations of LH showed a significant interaction (
p<0.05) between exposure and sampling period, with a greater LH decline dur
ing exposure to toluene than sham exposure. However, there was no concomita
nt effect on testosterone concentrations. The LH pulse frequency of women i
n the luteal phase showed a trend towards a significant interaction between
exposure and sampling period (p=0.06), with a greater decline in pulse fre
quency during exposure to toluene than sham exosure. There were no other si
gnificant effects of exposure to toluene.
Conclusions-Three hour exposure to 50 ppm toluene did not result in abnorma
l episodic LH or FSH secretion profiles, however, subtle effects on LH secr
etion in men and women in the luteal phase were found. The clinical relevan
ce of these effects is unclear, indicating the need for further study of re
productive function in exposed workers.