Effects of thiopental, ketamine, etomidate, propofol and midazolam on the production of adrenomedullin and endothelin-1 in vascular smooth muscle cells
Y. Hayashi et al., Effects of thiopental, ketamine, etomidate, propofol and midazolam on the production of adrenomedullin and endothelin-1 in vascular smooth muscle cells, RES COM M P, 103(3), 1999, pp. 325-331
Citations number
11
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
RESEARCH COMMUNICATIONS IN MOLECULAR PATHOLOGY AND PHARMACOLOGY
Endothelin-1 (ET-1) and adrenomedullin (AM) are potent vasoconstrictive and
vasodilative peptides. Vascular smooth muscle cells are one of the major p
roducing tissues of these peptides. We investigated effect of thiopental, k
etamine, etomidate, midazolam and propofol on the production of ET-1 and AM
in cultured rat aorta vascular smooth muscle cells. Rat cultured vascular
smooth muscle cells are used and the dose-dependent effect (10(-8)-10(-4) M
) of thiopental, ketamine, etomidate, midazolam, and propofol on the produc
tion of ET-1 and AM was examined. The concentartion of these peptides in th
e culture medium were measured by radioimmunoassay. Ketamine, etomidate, pr
opofol and midazolam, but not thiopental, decreased the production of ET-1
at a concentration of 10(-4) M and the effect of midazolam is the most pote
nt. Thiopental and etomidate produced small but significant increases in th
e production of AM at a concentration of 10(-4) M. Ketamine did not affect
the production, but propofol and midazolam decreased the production at 10(-
4) M and 10(-5) M, respectively. These results suggest that thiopental, ket
amine, etomidate, propofol and midazolam differentially affect the producti
on of ET-1 and AM in rat vascular smooth muscle cells.