BINDING OF ANTIBODIES AGAINST GM1 AND GD1B IN HUMAN PERIPHERAL-NERVE

Human dorsal root ganglia (DRG), and ventral and dorsal roots were imm unostained with rabbit antibodies recognizing GM1, GD1b, or both. Sera from rabbits immunized with GM1 or GD1b were separated in affinity co lumns into three fractions: Rab1, Rab2, and Rab3. Rab1 recognized only GM1, and Rab2 only GD1b; whereas Rab3 recognized both GM1 and GD1b, p resumably by binding to the terminal galactosy[beta 1-3N-acetylgalacto saminyl residue. Rab2 and Rab3 immunostained most of the nerve cell bo dies in the DRG and paranodal myelin of the ventral and dorsal roots, whereas Rab1 produced no significant immunostaining. These results sho w that GD1b is localized on the DRG neurons and the paranodal myelin o f human peripheral nerve. These places may be the binding sites for an ti-GD1b antibodies, including those cross-reactive with GM?, in the se ra from patients with autoimmune neuropathies. GM1 may be dispersed in human DRG and dorsal and ventral roots. (C) 1997 John Wiley & Sans, I nc.