K. Kemparaju et al., Purification and characterization of a platelet aggregation inhibitor acidic phospholipase A(2) from Indian saw-sealed viper (Echis carinatus) venom, TOXICON, 37(12), 1999, pp. 1659-1671
An acidic phospholipase A(2) (EC-I-PLA(2)) has been purified from the India
n saw-scaled viper (Echis carinatus) venom through a combination of column
chromatography and electrophoresis. EC-I-PLA(2) has a molecular weight of 1
6000 by SDS-PAGE. It was focussed between pH 4.2 and 4.8 by isoelectro focu
ssing. EC-I-PLA(2) was non-lethal to mice and devoid of neurotoxicity, myot
oxicity, anticoagulant activity and cytotoxicity. It induced mild oedema in
the foot pads of mice. The purified PLA(2) inhibited ADP, collagen and epi
nephrine induced human platelet aggregation and the inhibition was both dos
e and time dependent. (C) 1999 Elsevier Science Ltd. All rights reserved.