Purification and characterization of a platelet aggregation inhibitor acidic phospholipase A(2) from Indian saw-sealed viper (Echis carinatus) venom

Authors
Kemparaju, K Krishnakanth, TP Gowda, TV
Citation
K. Kemparaju et al., Purification and characterization of a platelet aggregation inhibitor acidic phospholipase A(2) from Indian saw-sealed viper (Echis carinatus) venom, TOXICON, 37(12), 1999, pp. 1659-1671
Citations number
32
Categorie Soggetti
Pharmacology & Toxicology
Journal title
TOXICON
ISSN journal
00410101 → ACNP
Volume
37
Issue
12
Year of publication
1999
Pages
1659 - 1671
Database
ISI
SICI code
0041-0101(199912)37:12<1659:PACOAP>2.0.ZU;2-#
Abstract
An acidic phospholipase A(2) (EC-I-PLA(2)) has been purified from the India n saw-scaled viper (Echis carinatus) venom through a combination of column chromatography and electrophoresis. EC-I-PLA(2) has a molecular weight of 1 6000 by SDS-PAGE. It was focussed between pH 4.2 and 4.8 by isoelectro focu ssing. EC-I-PLA(2) was non-lethal to mice and devoid of neurotoxicity, myot oxicity, anticoagulant activity and cytotoxicity. It induced mild oedema in the foot pads of mice. The purified PLA(2) inhibited ADP, collagen and epi nephrine induced human platelet aggregation and the inhibition was both dos e and time dependent. (C) 1999 Elsevier Science Ltd. All rights reserved.