Development and evaluation of an indirect ELISA for detection of exfoliative toxin ExhA, ExhB or ExhC produced by Staphylococcus hyicus

Authors
Citation
Lo. Andresen, Development and evaluation of an indirect ELISA for detection of exfoliative toxin ExhA, ExhB or ExhC produced by Staphylococcus hyicus, VET MICROB, 68(3-4), 1999, pp. 285-292
Citations number
14
Categorie Soggetti
Veterinary Medicine/Animal Health",Microbiology
Journal title
VETERINARY MICROBIOLOGY
ISSN journal
03781135 → ACNP
Volume
68
Issue
3-4
Year of publication
1999
Pages
285 - 292
Database
ISI
SICI code
0378-1135(19990831)68:3-4<285:DAEOAI>2.0.ZU;2-T
Abstract
Immunoblot analysis and enzyme-linked immunosorbent assay (ELISA) confirmed previous reports that the Staphylococcus hyicus exfoliative toxins ExhA an d ExhB are metalloproteins, and further indicated that ExhC is also a metal loprotein. An indirect ELISA. was developed for the detection of toxigenic strains as an alternative method to the use of phage typing for selection o f S. hyicus isolates to be used in autogenous vaccine against exudative epi dermitis in pigs. The indirect ELISA was evaluated by investigating the pre sence of toxin among a total of 655 S. hyicus isolates from 69 pig skin sam ples, one from each of the 69 pig herds with outbreak of exudative epidermi tis. Toxigenic S. hyicus were detected in 74% of the cases by ELISA. From e ach of the five cases, in which initially no toxigenic S. hyicus were found , a further 40 S. hyicus-like colonies were tested in ELISA. Testing of thi s number of colonies has a >99% probability of disclosing toxigenic S. hyic us. Toxin-producing isolates were found in only two of the five cases inves tigated. This may indicate the existence of one or more variants of the exf oliative toxin of S. hyicus that are not detected in the indirect ELISA or that S. hyicus may be displaced from lesions of exudative epidermitis. (C) 1999 Elsevier Science B.V. All rights reserved.