Application of aqueous hydrazine solution for beta-elimination of O-glycans from gastric mucin

Authors
Kisiel, D Gindzienski, A Gadek, A
Citation
D. Kisiel et al., Application of aqueous hydrazine solution for beta-elimination of O-glycans from gastric mucin, ACT BIOCH P, 46(3), 1999, pp. 753-757
Citations number
9
Categorie Soggetti
Biochemistry & Biophysics
Journal title
ACTA BIOCHIMICA POLONICA
ISSN journal
0001527X → ACNP
Volume
46
Issue
3
Year of publication
1999
Pages
753 - 757
Database
ISI
SICI code
0001-527X(1999)46:3<753:AOAHSF>2.0.ZU;2-Z
Abstract
O-Glycans from pig gastric mucin were released by beta-elimination in 0.2 M triethylamine and 50% aqueous hydrazine solution. The released glycan hydr azides were isolated using Centricon 10 separators, brought to their reduci ng form and reductive by labelled with p-aminobenzoic acid ethyl ester (ABE E). Labelled products were fractionated into neutral and acid fractions on a Bio-Gel P4 column, calibrated with a mixture of dextran oligosaccharides, labelled according to the same procedure.