Physical mapping of the serotonin 5-HT7 receptor gene (HTR7) to chromosome10 and pseudogene (HTR7P) to chromosome 12, and testing of linkage disequilibrium between HTR7 and autistic disorder

Citation
Jp. Lassig et al., Physical mapping of the serotonin 5-HT7 receptor gene (HTR7) to chromosome10 and pseudogene (HTR7P) to chromosome 12, and testing of linkage disequilibrium between HTR7 and autistic disorder, AM J MED G, 88(5), 1999, pp. 472-475
Citations number
28
Categorie Soggetti
Molecular Biology & Genetics
Journal title
AMERICAN JOURNAL OF MEDICAL GENETICS
ISSN journal
01487299 → ACNP
Volume
88
Issue
5
Year of publication
1999
Pages
472 - 475
Database
ISI
SICI code
0148-7299(19991015)88:5<472:PMOTS5>2.0.ZU;2-T
Abstract
The gene encoding the serotonin 5-HT7 receptor (HTR7) has been considered a s a candidate locus in several neuropsychiatric disorders, based on pharmac ological evidence and ligand-binding studies. After determining over 3 kb o f previously unpublished sequence from introns 1 and 2 of HTR7, a single ba se (C/T) polymorphism in the second intron of HTR7 was found. Allele-specif ic PCR was used to genotype the HTR7 marker in 53 trios consisting of subje cts with autistic disorder and both parents. Using the transmission disequi librium test (TDT), no evidence of preferential transmission of either alle le was found (TDT chi(2) 0.252, p = 0.602), Sequence data obtained from bot h intron 1 and intron 2 of HTR7, and from the 5-HT7 pseudogene (HTR7P), was used to confirm localization of HTR7 to 10q23 and HTR7P to 12p13 using rad iation hybrid analyses.. (C) 1999 Wiley-Liss, Inc.