Physical mapping of the serotonin 5-HT7 receptor gene (HTR7) to chromosome10 and pseudogene (HTR7P) to chromosome 12, and testing of linkage disequilibrium between HTR7 and autistic disorder
Jp. Lassig et al., Physical mapping of the serotonin 5-HT7 receptor gene (HTR7) to chromosome10 and pseudogene (HTR7P) to chromosome 12, and testing of linkage disequilibrium between HTR7 and autistic disorder, AM J MED G, 88(5), 1999, pp. 472-475
The gene encoding the serotonin 5-HT7 receptor (HTR7) has been considered a
s a candidate locus in several neuropsychiatric disorders, based on pharmac
ological evidence and ligand-binding studies. After determining over 3 kb o
f previously unpublished sequence from introns 1 and 2 of HTR7, a single ba
se (C/T) polymorphism in the second intron of HTR7 was found. Allele-specif
ic PCR was used to genotype the HTR7 marker in 53 trios consisting of subje
cts with autistic disorder and both parents. Using the transmission disequi
librium test (TDT), no evidence of preferential transmission of either alle
le was found (TDT chi(2) 0.252, p = 0.602), Sequence data obtained from bot
h intron 1 and intron 2 of HTR7, and from the 5-HT7 pseudogene (HTR7P), was
used to confirm localization of HTR7 to 10q23 and HTR7P to 12p13 using rad
iation hybrid analyses.. (C) 1999 Wiley-Liss, Inc.