High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells

Authors
Duncan, RR Don-Wauchope, AC Tapechum, S Shipston, MJ Chow, RH Estibeiro, P
Citation
Rr. Duncan et al., High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells, BIOCHEM J, 342, 1999, pp. 497-501
Citations number
17
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL JOURNAL
ISSN journal
02646021 → ACNP
Volume
342
Year of publication
1999
Part
3
Pages
497 - 501
Database
ISI
SICI code
0264-6021(19990915)342:<497:HSFVTI>2.0.ZU;2-2
Abstract
Adrenal chromaffin cells are commonly used in studies of exocytosis. Progre ss in characterizing the molecular mechanisms has been slow, because no sim ple, high-efficiency technique is available for introducing and expressing heterologous cDNA in chromaffin cells. Here we demonstrate that Semliki For est virus (SFV) vectors allow high-efficiency expression of heterologous pr otein in chromaffin cells.