QUANTITATIVE-ANALYSIS OF ENZYME-MEDIATED HYDROLYTIC PROCESSES AND THEIR PRODUCTS USING HPLC AND NMR TECHNIQUES

Second derivative of the UV spectra of 2-(4-alkoxybenzyl)cyclopentan-1 -ols and their acetates were used for the quantitative analysis of rea ction kinetics of enzyme-mediated hydrolytic processes, To determine t he enantiomeric purity of the products using non-chiral HPLC columns. their diastereoisomeric esters of 3,3,3-trifluoro-2-methoxy-2-phenylpr opanoic acid (MTPA) were prepared. The absolute configuration of the p roducts was established using a combination of the results of a HPLC a nalysis and H-1/F-19-NMR measurements of the diastereoisomeric MTP est ers. The analytical method described consists of an easy routine HPLC analysis which can sometimes be used as a quick on-line analysis of th e reaction kinetics and as a quick stepwise analysis of the optical pu rity and the absolute configuration of the products.