The viral enhancer Blankophor BBH was applied by air to gypsy moth, Lymantr
ia dispar, populations in two formulations of gypsy moth nucleopolyhedrovir
us to determine if the enhancer could compensate for reduced rates of virus
. Larval mortality due to virus in plots treated with a molasses-based form
ulation at 2.5 x 10(11) polyhedral inclusion bodies (PIB)/ha (one-fourth th
e United States Department of Agriculture (USDA) Forest Service recommended
rate) was 25% greater than in untreated plots. There was no increase in la
rval mortality in plots treated with an experimental wettable-powder formul
ation at 5 x 10(11) PIB/ha (one-half the recommended virus rate) compared t
o untreated plots but defoliation was 50% lower. The addition of 0.5% (W:V)
Blankophor BBH did not increase the efficacy of either formulation. The vo
lume of spray deposited onto leaf surfaces averaged 16.6 and 40.0 nl/cm(2)
for the molasses-based formulation and 6.9 and 13.7 nl/cm(2) for the wettab
le-powder formulation with and without enhancer, respectively. A separate l
aboratory experiment in which 0.5-mu l droplets containing low (200 PIB/mu
l) concentrations of virus with varying concentrations of Blankophor BBH we
re consumed by End-stage gypsy moth larvae demonstrated that viral enhancem
ent increased with increasing concentration of Blankophor BBH and that a co
ncentration of 0.3% was required to cause >90% larval mortality. This conce
ntration in a 0.5-mu l droplet corresponds to 1.5 mu g of Blankophor BBH, w
hich is proposed as an estimate of the dose required to cause >90% mortalit
y in End-stage gypsy moth larvae. Based on the amount of spray deposited in
the held test, only 0.03-0.2 mu g of Blankophor BBH/cm(2) of leaf surface
was deposited, which was substantially below the proposed amount required f
or enhancement. The LC50 was 2.8 times greater when oak rather than lettuce
was used as a host substrate without Blankophor BBH and 1.9 times greater
with Blankophor BBH. The enhancer reduced LC50 values 213- and 314-fold on
lettuce and oak, respectively. (C) 1999 Academic Press.