A comparative study of estrogen receptors alpha and beta in the rat uterus

The uterus is an important target organ for steroid hormones. The effects o f these hormones are mediated via specific receptors. The aim of this study was to compare the expression, distribution, and regulation of estrogen re ceptor (ER) alpha and beta in the rat uterus in order to establish possible different biological roles for the two receptor forms. Ovariectomized rats were treated with either estradiol (E-2), progesterone (P-4), or combinati ons of these for 24 or 48 h. The mRNA levels were measured by solution hybr idization. Distribution of the mRNAs and receptor proteins was detected by in situ hybridization and immunohistochemistry. The results showed that ER alpha is the dominating subtype in the rat uterus. E-2 seemed to increase t he ER alpha mRNA level in the glandular and luminal epithelium, but it caus ed a decrease of the immunostaining intensity in the glandular epithelium. P-4 reduced ER alpha expression in luminal epithelium whereas no effect was seen in the glandular epithelium. E-2 or P-4 did not alter the expression of ER beta, on either the mRNA or protein level. In conclusion, the distrib ution and regulation of ER alpha and ER beta differ in the different compar tments of the rat uterus. The complex uterine responses to E-2 and P-4 are directly or indirectly mediated by differential cell-specific expression of their receptors. The low expression in the uterus and the limited regulati on by gonadal steroids in this study suggest that ER beta probably plays a minor role in the regulation of uterine physiology.