Androgen-dependent nitric oxide release in rat penis correlates with levels of constitutive nitric oxide synthase isoenzymes

Androgens are known to influence penile erection and nitric oxide synthase (NOS) activity in cavernosal tissue homogenates. The present study was an a ssessment of the effects of castration and androgen replacement on the in v ivo release of nitric oxide (NO), and of the simultaneously recorded intrac avernosal pressure (ICP) changes elicited by electrostimulation of the cave rnosal nerves (SCN) in the anesthetized rat. The extracellular levels of NO in the corpora were monitored electrochemically using porphyrin microsenso rs. The content of NOS isoenzymes in corporal homogenates was determined by immunoblotting. The responses of castrated rats with or without testostero ne (T) implants were compared to those of intact animals. Castration virtua lly abolished both the NO and the ICP responses to SCN. There was a concomi tant significant decrease in the content of both the neuronal (nNOS) and th e endothelial (eNOS) isoenzymes in the cavernosal tissue. All these effects of castration were prevented by T replacement. The NO response to SCN was positively correlated with the levels of nNOS and eNOS, especially when the values of the two isoforms were added (r = 0.71, P < 0.001). These data su ggest that the facilitatory action of androgens on penile erection involves the up-regulation of both constitutive NOS isoenzymes in the corpora caver nosa.