The newly established GUS expression bioassay on the callus extracts of 22
species of plants revealed that the methanol extract of Phytolacca american
a callus had the most potent inhibitory activity against agrobacterial plan
t transformation. A triterpene glycoside phytolaccoside B was isolated from
the extract as a genuine plant transformation inhibitor having neither ant
iagrobacterial nor phytotoxic activity. This compound is promising for use
as a biochemical probe for studies on the plant transformation mechanism.