Topical application of neurotrophin-3 attenuates ischemic brain injury after transient middle cerebral artery occlusion in rats

Authors
Zhang, WR Kitagawa, H Hayashi, T Sasaki, C Sakai, K Warita, H Shiro, Y Suenaga, H Ohmae, H Tsuji, S Itoh, T Nishimura, O Nagasaki, H Abe, K
Citation
Wr. Zhang et al., Topical application of neurotrophin-3 attenuates ischemic brain injury after transient middle cerebral artery occlusion in rats, BRAIN RES, 842(1), 1999, pp. 211-214
Citations number
17
Categorie Soggetti
Neurosciences & Behavoir
Journal title
BRAIN RESEARCH
ISSN journal
00068993 → ACNP
Volume
842
Issue
1
Year of publication
1999
Pages
211 - 214
Database
ISI
SICI code
0006-8993(19990918)842:1<211:TAONAI>2.0.ZU;2-S
Abstract
In order to examine the effect of neurotrophin-3 (NT-3) on ischemic brain i njury, NT-3 was topically applied to brain surface just after 90 min of mid dle cerebral artery occlusion (MCAO) in rats. NT-3 significantly reduced th e infarct size at 24 h of reperfusion. Terminal deoxynucleotidyl transferas e-mediated dUTP-biotin in situ nick labeling (TUNEL) staining and immunohis tochemical study for caspase-3 and heat shock protein 72 (HSP72) showed tha t NT-3 treatment decreased the number of cells with DNA fragmentation and c aspase-3 and HSP72 expressions. These data suggest that NT-3 protects neuro nal cells from ischemic injury, and it is possibly associated with inhibiti on of DNA fragmentation. (C) 1999 Elsevier Science B.V. All rights reserved .