Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8) DNA sequences are absent in leukapheresis products and ex vivo expanded CD34(+) cells from multiple myeloma patients

Authors
De Greef, C Van de Voorde, W Bakkus, M Corthals, J Heirman, C Schots, R Lacor, P Van Camp, B Van Riet, I
Citation
C. De Greef et al., Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8) DNA sequences are absent in leukapheresis products and ex vivo expanded CD34(+) cells from multiple myeloma patients, BR J HAEM, 106(4), 1999, pp. 1033-1036
Citations number
12
Categorie Soggetti
Hematology,"Cardiovascular & Hematology Research
Journal title
BRITISH JOURNAL OF HAEMATOLOGY
ISSN journal
00071048 → ACNP
Volume
106
Issue
4
Year of publication
1999
Pages
1033 - 1036
Database
ISI
SICI code
0007-1048(199909)106:4<1033:KSH(DS>2.0.ZU;2-X
Abstract
Recently it was reported that Kaposi's sarcoma-associated herpesvirus (KSHV /HHV-8) infects bone marrow (BM) dendritic cells (DC) in multiple myeloma ( MM) patients and therefore might play a role in MM development, Because of the use of myeloid growth factors like GM-CSF and G-CSF for the mobilizatio n of peripheral blood progenitor cells (PBPC), the subsequent increase of D C precursors might imply a risk for KSHV contamination in PBPC grafts. Ther efore, in this study leukapheresis products and ex vivo cultured CD34(+) ce ll suspensions were analysed, KSHV DNA could not be amplified in any of the m.