Isolation and characterization of macaque dendritic cells from CD34(+) bone marrow progenitors

We have developed a method for isolating and characterizing pigtailed macaq ue dendritic cells (DCs) generated from CD34(+) bone marrow (BM) progenitor s based on methods previously developed for isolating human DCs. Macaque DC s displayed a characteristic morphology and were potent stimulators of allo geneic T cell proliferation. They expressed a set of DC-associated markers, such as MHC class II, CD1a, CD4, CD11a, CD40, CD58, CD80, CD83, CD86, and CXCR4. Macaque DCs, as well as peripheral blood CD4(+) T cells, were highly susceptible to HIV-2 infection, as detected by DNA-PCR. The expression of HIV-2 in macaque DCs was downregulated by treatment with the beta-chemokine RANTES. Macaque DCs will be useful for defining the in vivo role of DCs in HIV pathogenesis and for optimizing and testing peptide-DC vaccines or tol erizing regimens. (C) 1999 Academic Press.