COMBINED IMMUNOSTAINING AND COOMASSIE-BRILLIANT-BLUE STAINING OF POLYVINYLIDENE DIFLUORIDE MEMBRANES WITHOUT ORGANIC-SOLVENT

Authors
HOUEN G BRUUN L BARKHOLT V
Citation
G. Houen et al., COMBINED IMMUNOSTAINING AND COOMASSIE-BRILLIANT-BLUE STAINING OF POLYVINYLIDENE DIFLUORIDE MEMBRANES WITHOUT ORGANIC-SOLVENT, Electrophoresis, 18(5), 1997, pp. 701-705
Citations number
9
Categorie Soggetti
Biochemical Research Methods
Journal title
Electrophoresis → ACNP
ISSN journal
01730835
Volume
18
Issue
5
Year of publication
1997
Pages
701 - 705
Database
ISI
SICI code
0173-0835(1997)18:5<701:CIACSO>2.0.ZU;2-Q
Abstract
A method for staining proteins on polyvinylidene difluoride membranes without using organic solvent is described. The method uses preblockin g of the membrane with either Tween 20 or polyethylene glycol followed by staining with 0.01% Coomassie Brilliant Blue. No destaining of the membrane is needed afterwards. Preblocking with polyethylene glycol i s compatible with microsequencing while Tween 20 leads to very low ini tial yields. Preblocking with Tween 20 has the additional advantage of allowing immunostaining followed by Coomassie Brilliant Blue staining for total protein on the same membrane.