Immortalized kidney epithelial cells as tools for hormonally regulated iontransport studies

The development of transgenic mice carrying the simian virus-400 large T an tigen gene or the temperature-sensitive simian virus-40 large T antigen gen e, either alone or placed under the control of the 5'-regulatory regions of tissue-specific or ubiquitous genes, has permitted the production of diffe rentiated, polarized kidney epithelial cells. This review covers the immort alized cell lines issued from the various parts of the renal tubule and, in particular, the recently established collecting duct cell lines that have been used as ex-vivo cell models to analyze the regulation of ion transport processes by hormones, Curr Opin Nephrol Hypertens 8:581-587. (C) 1999 Lip pincott Williams & Wilkins.