Activity-dependent enhancement of hyperpolarizing and depolarizing gamma-aminobutyric acid (GABA) synaptic responses following inhibition of GABA uptake by tiagabine
Mf. Jackson et al., Activity-dependent enhancement of hyperpolarizing and depolarizing gamma-aminobutyric acid (GABA) synaptic responses following inhibition of GABA uptake by tiagabine, EPILEPSY R, 37(1), 1999, pp. 25-36
The effects of the gamma-aminobutyric acid (GABA) uptake blocker tiagabine
on isolated inhibitory postsynaptic potentials (IPSPs) were examined in CA1
pyramidal cells of the rat hippocampal slice preparation. The IPSPs were e
licited by either single stimuli or by high frequency (100 Hz, 200 ms) stim
ulation (HFS) of inhibitory interneurons. Bath applied tiagabine (20 mu M)
produced little or no increase in the amplitude of IPSPs evoked by low (30-
50 mu A) or high (200-400 mu A) intensity single stimuli. Only the duration
of IPSPs evoked by high intensity stimuli was substantially prolonged by t
iagabine, the time integral of the hyperpolarizing response being increased
3.2-fold. HFS elicited much larger fast and slow IPSPs than a single stimu
lus. In addition, with increments in the intensity (80-550 mu A) of HFS, a
GABA, receptor-mediated depolarizing response of progressively larger ampli
tude appeared between, and overlapped with, the fast and slow hyperpolarizi
ng components of the IPSP. Tiagabine application markedly increased the GAB
A-mediated responses evoked by both low and high intensity HFS. Increasing
the intensity of HFS enhanced the drug effect. Thus, measurements of the ti
me integral of evoked responses showed that with weak (60 mu A) HFS, tiagab
ine caused a 3.6-fold increase in the area of hyperpolarization while, in c
ontrast, with strong (530 mu A) HFS, tiagabine produced a 13.5-fold increas
e in the depolarizing actions of GABA. Our results suggest that tiagabine,
a therapeutically effective anticonvulsant, may paradoxically increase, thr
ough a GABA(A) receptor-mediated mechanism, neuronal depolarization during
the high frequency discharge of neurons involved in epileptiform activity.
(C) 1999 Elsevier Science B.V. All rights reserved.