The effects of hydrostatic pressure on the conformation of plasminogen

Authors
Kornblatt, JA Kornblatt, MJ Clery, C Balny, C
Citation
Ja. Kornblatt et al., The effects of hydrostatic pressure on the conformation of plasminogen, EUR J BIOCH, 265(1), 1999, pp. 120-126
Citations number
45
Categorie Soggetti
Biochemistry & Biophysics
Journal title
EUROPEAN JOURNAL OF BIOCHEMISTRY
ISSN journal
00142956 → ACNP
Volume
265
Issue
1
Year of publication
1999
Pages
120 - 126
Database
ISI
SICI code
0014-2956(199910)265:1<120:TEOHPO>2.0.ZU;2-E
Abstract
Plasminogen undergoes a large conformational change when it binds 6-aminohe xanoate. Using ultraviolet absorption spectroscopy and native PAGE, we show that hydrostatic pressure brings about the same conformational change. The volume change for this conformational change is -33 mL.mol(-1). Binding of ligand and hydrostatic pressure both cause the protein to open up to expos e surfaces that had previously been buried in the interior.