Effect of high-fat diet on lypolisis in isolated adipocytes from visceral and subcutaneous WAT

Variations in total energy intake and composition of daily food play an imp ortant role in the regulation of metabolic processes and so, in the control of body weight. This study was designed in order to investigate the effect of a high-fat diet on lipolysis in isolated adipocytes. For this purpose, fourteen Wistar rats were divided into two groups and fed either a standard -fat diet or a high-fat diet ad libitum for 7 weeks. Adipocytes were prepar ed from fat pads by collagenase digestion and incubated in vitro in the abs ence or presence of various lipolytic agents. Lipolysis was measured by the release of glycerol into the medium during 90 min of incubation. We observ ed that a high amount of fat in the diet induced an enlargement of adipose tissue, which was accompanied by a reduction of beta-adrenergic agonist-ind uced lipolysis, that could be due to a loss of beta(1) and beta(3)-adrenoce ptor number or to alterations of their coupling to adenylate-cyclase throug h the guanine nucleotide regulatory protein. New data about regional differ ences were provided by comparing two adipose locations (subcutaneous and vi sceral).