Molecular cloning of cDNA encoding a cyclin-selective ubiquitin carrier protein (E2-C) from Curassius auratus (goldfish) and expression analysis of the cloned gene

Destruction of cyclin B is required for exit from mitosis and meiosis, A cy clin-specific ubiquitinating system, including cyclin-selective ubiquitin c arrier protein (E2-C), is thought to be responsible for cyclin B destructio n. Here we present the cloning, sequencing and expression analaysis of gold fish, Carassins auratus, E2-C which encodes the cyclin-selective ubiquitin carrier protein from goldfish ovary. The cloned cDNA is 677 bp long and enc odes 172 amino acids, The deduced amino acid sequence is highly homologous to E2-C from other species. Recombinant goldfish E2-C possesses ubiquitinat ing activity against cyclin B, The expression of mRNA for E2-C was similar to that of mRNA for cyclin B, occurring at very high level in the ovary. Th e similarity of the expression pattern of E2-C and cyclin B suggests that E 2-C mediates a cyclin-specific ubiquitination, (C) 1999 Federation of Europ ean Biochemical Societies.