Recruitment of the SWI/SNF chromatin remodeling complex by transcriptionalactivators

SWI/SNF is a chromatin remodeling complex that facilitates expression of a number of yeast genes. Here we demonstrate that SWI/SNF can be recruited fr om yeast nuclear extracts by a transcriptional activator. Recruitment is de pendent on an activation domain but not on promoter sequences, TBP, or RNA polymerase II holoenzyme. We also show that acidic activation domains can t arget SWI/SNF remodeling activity. These results demonstrate that SWI/SNF a ctivity can be targeted by gene-specific activators and that this recruitme nt can occur independently of Pol II holoenzyme.