M. Partridge et al., Location of candidate tumour suppressor gene loci at chromosomes 3p, 8p and 9p for oral squamous cell carcinomas, INT J CANC, 83(3), 1999, pp. 318-325
To help define the location of tumour suppressor genes implicated in the pa
thogenesis of oral squamous cell carcinoma (SCC), we have used microsatelli
te assay and restriction fragment: length polymorphism (RFLP) analysis to s
creen 48 primary SCC for allelic imbalance (Al) with 32 polymorphic markers
at chromosome 3p, and prepared a detailed deletion map. The finding of a h
igh frequency of Al at specific regions, together with the presence of mult
iple small interstitial deletions involving these loci, identifies 5 areas
at this chromosome arm that may harbour tumour suppressor genes. No sequenc
e aberrations affecting the von Hippel Lindau (VHL) and fragile histidine t
riad (FHIT) genes, which reside within the candidate tumour suppressor gene
areas at this chromosome arm, were identified. A more limited analysis of
polymorphic sequences at 8p and 9p supports the existence of at least 2 are
as that harbour tumour suppressor genes at 8p and evidence that additional
targets for deletion reside centromeric and telomeric to the p16 gene at 9p
21. (C) 1999 Wiley-Liss, Inc.