Relevance of matrix effect in determination of biogenic amines in plaice (Pleuronectes platessa) and whiting (Merlangus merlangus)

Spoilage can be evaluated by separating and determining biogenic amines by various techniques, notably high-performance liquid chromatography. Previou s studies have not taken into account how the muscle tissue matrix affects the assay. We demonstrate a matrix effect in plaice and whiting and show th at it changes during spoilage. This effect should be taken into account whe n plotting regression lines relating the quantity of amine to the biogenic amine/internal standard ratio.