Spore peptidoglycan structure in a cwlD dacB double mutant of Bacillus subtilis

Bacillus subtilis cwlD and dacB mutants produce spore peptidoglycan (PG) wi th increased cross-linking but with little change in spore core hydration c ompared to the wild type. A cwlD dacB double mutant produced spores with a two- to fourfold greater increase in PG cross-linking and novel muropeptide s containing glycine residues but no significant changes in spore resistanc e or core hydration.