Identification and confirmation of 3-hydroxy metabolite of ketoprofen in camels by gas chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy

The metabolites of ketoprofen were investigated in five camels following in travenous administration of a dose of 2.0 mg/kg body weight. Two metabolite s were identified. The first one was purified with thin-layer chromatograph y. It was identified by gas chromatography-mass spectrometry (GC-MS) in com parison with authenticated reference standard and was found to be hydroxyke toprofen due to reduction of the ketone group of ketoprofen. The second met abolite was purified by high-performance Liquid chromatography. It was iden tified with GC-MS and nuclear magnetic resonance spectroscopy as 3-hydroxyb enzolketoprofen resulting from oxidation of the aromatic ring. (C) 1999 Pub lished by Elsevier Science B.V. All rights reserved.