M. Triantafilou et al., Involvement of beta(2)-microglobulin and integrin alpha(v)beta(3) molecules in the coxsackievirus A9 infectious cycle, J GEN VIROL, 80, 1999, pp. 2591-2600
It is becoming apparent that many viruses employ more than one cell surface
molecule for their attachment and cell entry. In this study, we have teste
d the role of integrin alpha(v)beta 3 and MHC class I molecules in the coxs
ackievirus A9 (CAV-9) infectious cycle. Binding experiments utilizing CHO c
ells transfected and expressing human integrin alpha(v)beta 3, revealed tha
t CAV-9 particles were able to bind to cells, but did not initiate a produc
tive cell infection, Antibodies specific for integrin alpha(v)beta 3 molecu
les significantly reduced CAV-9 infection in susceptible cell lines. Moreov
er, MAbs specific for beta(2)-microglobulin (beta(2)-m) and MHC class I mol
ecules completely inhibited CAV-9 infection. To assess the effect of these
antibodies on virus binding, we analysed CAV-9 binding by Row cytometry in
the presence of beta(2)-m- or integrin alpha(v)beta 3-specific antibodies,
The results showed a reduction in CAV-9 binding in the presence of integrin
alpha(v)beta 3-specific antibodies while there was no reduction in the pre
sence of beta(2)-m-specific MAb, Taken together, these data suggest that in
tegrin alpha(v)beta 3 is required for CAV-9 attachment but is not sufficien
t for cell entry, while beta(2)-m, although not directly involved in CAV-9
binding, plays a post-attachment role in the CAV-9 infectious process, poss
ibly being involved in virus entry.