Complete sequence of RNA 1 and the presence of tRNA-like structures in allRNAs of Potato mop-top virus, genus Pomovirus

The complete nucleotide sequence (6043 nt) of RNA 1 from Potato mop-top vir us (PMTV-Sw), the type member of the genus Pomovirus, was determined, The f irst (5'-terminal) open reading frame (ORF 1) encodes a predicted protein o f 148 kDa, ORF 2 extends through the opal stop codon of ORF 1 producing a p redicted readthrough protein of 206 kDa which resembles the RNA-dependent R NA polymerases (RdRp) of other fungal-transmitted viruses. It includes a me thyltransferase, a helicase and a GDD RdRp motif, respectively. Phylogeneti c analyses of RdRps indicated that PMTV is most closely related to Beet soi l-borne virus (genus Pomovirus), Broad bean necrosis virus (genus Pomovirus ) and Soil-borne wheat mosaic virus (genus Furovirus), and is more distantl y related to the other viruses of the former furovirus group, The 5' and 3' termini of RNA 1 in PMTV contained untranslated regions (UTR) of 114 nt an d 489 nt, respectively, The 3'-UTR of RNA 1 contained a tRNA-like structure , which has previously been reported in the 3'-UTR of RNA 2 but not RNA 3. However, in this study, the tRNA-like structure was also found in the 3'-UT R of RNA 3, which confirms its presence in the 3'-UTRs of all three RNAs of PMTV.