Tissue microdissection is potentially one of the most useful techniques in
molecular pathology, Laser-assisted microdissection has been developed to p
rocure precisely the cells of interest in a tissue specimen, in a rapid and
practical manner, Together with multiplex molecular approaches, it is now
feasible to study genetic alterations and isolate genes and proteins in def
ined cell populations from complex normal and diseased tissues. The fundame
ntal advantage of this technique is the possibility of capturing single cel
ls from which high-quality DNA and mRNA can be isolated for analysis of seq
uence and quantitation of expression, Moreover, the integration of laser-as
sisted microdissection and proteomic analysis could identify novel protein
markers for disease. The advent of laser-assisted microdissection is likely
to have a profound impact on molecular pathology. Copyright (C) 1999 John
Wiley & Sons, Ltd.