S. Salgueiro et R. Hull, Comparison of pea enation mosaic virus (PEMV) isolates: Sequence of coat protein and aphid transmission protein genes of a European isolate, J PHYTOPATH, 147(7-8), 1999, pp. 391-396
Citations number
17
Categorie Soggetti
Plant Sciences
Journal title
JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT
Pea enation mosaic virus (PEMV) is a bipartite virus, transmitted mechanica
lly and by aphids in the circulative manner. Virus capsid proteins, coded b
y RNA-I are involved in aphid transmissibility. Using an antiserum against
a European (UK) aphid-transmissible isolate, the capsid proteins of six PEM
V isolates, each aphid-transmissible or non-transmissible, were compared us
ing Western blots. All isolates have a major coat protein of approximately
22 kDa, but also additional proteins. RNA-1 complementary DNAs correspondin
g to ORFs 4 and 5 of isolate SP were cloned and sequenced. This had 95% hom
ology at the nucleotide level and a 96% homology at the amino acid level wi
th the sequence of a North American isolate. Most amino acid differences we
re in the region corresponding to ORF 5, some of which could result in pote
ntial changes in the conformation of the protein. This is the first report
on the sequence of an European PEMV isolate and the first direct comparison
of viral capsid complexity in different PEMV isolates.