Modified polymerase chain reaction and selective media methods for detection of Helicobacter pylori in pork loin chops

Citation
Vj. D'Costa et al., Modified polymerase chain reaction and selective media methods for detection of Helicobacter pylori in pork loin chops, J RAPID M A, 7(2), 1999, pp. 81-94
Citations number
29
Categorie Soggetti
Food Science/Nutrition
Journal title
JOURNAL OF RAPID METHODS AND AUTOMATION IN MICROBIOLOGY
ISSN journal
10603999 → ACNP
Volume
7
Issue
2
Year of publication
1999
Pages
81 - 94
Database
ISI
SICI code
1060-3999(199908)7:2<81:MPCRAS>2.0.ZU;2-K
Abstract
Selective media, and a modified polymerase chain reaction (PCR) were evalua ted for use in the detection and enumeration of Helicobacter pylori in pork loin chops. The chops were treated with tap water, or H. pylori-ATCC 43504 , packaged in polyvinyl chloride (PVC) film (7 days), or vacuum packaged (2 4 days), stored at 2C, and analyzed for survival of H. pylori. Modifying th e amplification cycles and annealing temperature of the PCR resulted in det ection of H. Pylori on inoculated chops. Helicobacter pylori survived for 5 , and 10 days on the PVC, and vacuum packaged chops, respectively. Skirrow agar was the most effective medium, when compared to blood agar, and egg yo lk emulsion agar.