The length of the polyoxyethylene chain in the Triton X detergents modulates the apparent activation of neurosteroid sulfatase in bovine brain

Authors
Park, IH Han, BK Cho, SJ Jo, DH
Citation
Ih. Park et al., The length of the polyoxyethylene chain in the Triton X detergents modulates the apparent activation of neurosteroid sulfatase in bovine brain, J STEROID B, 70(1-3), 1999, pp. 97-100
Citations number
14
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
ISSN journal
09600760 → ACNP
Volume
70
Issue
1-3
Year of publication
1999
Pages
97 - 100
Database
ISI
SICI code
0960-0760(199907/08)70:1-3<97:TLOTPC>2.0.ZU;2-4
Abstract
The effect of the Triton X series on the solubilization and enzyme activity of neurosteroid sulfatase (NSS) in the bovine midbrain was investigated. T riton X-100 and X165 stimulated NSS activity in the bovine midbrain, while Triton X-305 did not. This apparent activation was attributed to the action of the detergents, and not to the latency of the enzyme or the removal of some inhibitory substance from the microsomes. The maximum stimulation was obtained when the length of the polyoxyethylene chain of the detergent was 16. (C) 1999 Elsevier Science Ltd. All rights reserved.