A NOVEL SOLID SUPPORT FOR SYNTHESIS OF 3'-PHOSPHORYLATED CHIMERIC OLIGONUCLEOTIDES CONTAINING INTERNUCLEOSIDIC METHYL PHOSPHOTRIESTER AND METHYLPHOSPHONATE LINKAGES
A. Guzaev et H. Lonnberg, A NOVEL SOLID SUPPORT FOR SYNTHESIS OF 3'-PHOSPHORYLATED CHIMERIC OLIGONUCLEOTIDES CONTAINING INTERNUCLEOSIDIC METHYL PHOSPHOTRIESTER AND METHYLPHOSPHONATE LINKAGES, Tetrahedron letters, 38(22), 1997, pp. 3989-3992
A novel solid phase synthesis of 3'-phosphorylated oligonucleotides is
described. The chain assembly is carried out by phosphoramidite strat
egy on solid support 2, which allows a mild and fast release of the ol
igonucleotide in solution. The applicability of the method is demonstr
ated by preparation of 3'-phosphorylated chimeric oligonucleotides con
taining methyl phosphotriester and methyl phosphonate internucleosidic
linkages. (C) 1997 Elsevier Science Ltd.