Ascaris haemoglobin is a nitric oxide-activated 'deoxygenase'

The parasitic nematode Ascaris lumbricoides infects one billion people worl dwide. Its perienteric fluid contains an octameric haemoglobin(1-3) that bi nds oxygen nearly 25,000 times more tightly than does human haemoglobin(4,5 ). Despite numerous investigations, the biological function of this molecul e has remained elusive. The distal haem pocket contains a metal, oxygen and thiol(6), all of which are known to be reactive with nitric oxide. Here we show that Ascaris haemoglobin enzymatically consumes oxygen in a reaction driven by nitric oxide, thus keeping the perienteric fluid hypoxic. The mec hanism of this reaction involves unprecedented chemistry of a haem group, a thiol and nitric oxide. We propose that Ascaris haemoglobin functions as a 'deoxygenase', using nitric oxide to detoxify oxygen. The structural and f unctional adaptations of Ascaris haemoglobin suggest that the molecular evo lution of haemoglobin can be rationalized by its nitric oxide related funct ions.