Base modified oligodeoxynucleotides. II. Increase of stability to nucleases by 5-alkyl-, 5-(1-alkenyl)- and 5-(1-alkynyl)-pyrimidines

Authors
Otvos, L Sagi, J Sagi, G Szemzo, A
Citation
L. Otvos et al., Base modified oligodeoxynucleotides. II. Increase of stability to nucleases by 5-alkyl-, 5-(1-alkenyl)- and 5-(1-alkynyl)-pyrimidines, NUCLEOS NUC, 18(9), 1999, pp. 1929-1933
Citations number
8
Categorie Soggetti
Biochemistry & Biophysics
Journal title
NUCLEOSIDES & NUCLEOTIDES
ISSN journal
07328311 → ACNP
Volume
18
Issue
9
Year of publication
1999
Pages
1929 - 1933
Database
ISI
SICI code
0732-8311(1999)18:9<1929:BMOIIO>2.0.ZU;2-M
Abstract
The effect of pyrimidine base substitution on the sensitivity of oligonucle otides to nucleases has been studied with two series of self complementary deoxyoligonucleotides containing n-alkyl, n-(1-alkenyl)-or n-(1-alkynyl) gr oups at C5 of pyrimidines, (dA-r(5)dU)(10) and (dG-r(5)dC)(6). The rate of hydrolysis by snake venom phosphodiesterase and in human serum decreased wi th increasing length and unsaturation of the substituent.