Supplementation with fruit and vegetable extracts may decrease DNA damage in the peripheral lymphocytes of an elderly population

Fruit and vegetable consumption has been heralded for its ability to decrea se the overall risk of developing cancer and other diseases. Mounting evide nce supports the beneficial nature of antioxidants, carotenoids, and other phytonutrients found in fruits and vegetables. One proposed mechanism of an tioxidant protection is the shielding of cellular DNA from oxidative damage and therefore mutations. This may be especially helpful in older populatio ns. We tested the concept that a daily regimen of supplementation with frui t and vegetable extracts (JuicePlus(TM)) would reduce the amount of DNA dam age in the peripheral lymphocytes of the elderly. In a blind study, a group of twenty elderly volunteers (mean age= 68) were given supplements twice d aily for 80 days with blood samples drawn before and after intervention. Th ese samples were compared using the comet assay, a technique that quantifie s DNA damage to individual nuclei. Each sample was tested in triplicate, an d tail moment data was collected from over 200 comets per sample. Paired t- test analysis revealed a highly significant (p<0.0001) decrease in measured DNA damage between pre (13.24+/-2.77) and post (4.41+/-2.76) treatment tai l moment. Screening of test subjects' personal data showed no apparent rela tionship between age, sex, or smoking. In this initial study, we conclude t hat a daily course of fruit and vegetable extract supplementation may reduc e the level of DNA damage found in the peripheral lymphocytes of seniors. ( C) 1999 Elsevier Science Inc.