Plants require roots to supply water, nutrients and oxygen for growth. The
spatial distribution of roots in relation to the macropore structure of the
soil in which they are growing influences how effective they are at access
ing these resources. A method for quantifying root-macropore associations f
rom horizontal soil sections is illustrated using two black vertisols from
the Darling Downs, Queensland, Australia. Two-dimensional digital images we
re obtained of the macropore structure and root distribution for an area 55
x 55 mm at a resolution of 64 mu m. The spatial distribution of roots was
quantified over a range of distances using the K-function. In all specimens
, roots were shown to be clustered at short distances (1-10 mm) becoming mo
re random at longer distances. Root location in relation to macropores was
estimated using the function describing the distance of each root to the ne
arest macropore. From this function, a summary variable, termed the macropo
re sheath, was defined. The macropore sheath is the distance from macropore
s within which 80% of roots are located. Measured root locations were compa
red to random simulations of root distribution to establish if there was a
preferential association between roots and macropores. More roots were foun
d in and around macropores than expected at random.