Sera from 200 randomly selected individuals living in Karksi Nuia, south Es
tonia, near an area endemic for tick-borne encephalitis and Lyme borreliosi
s (LB), were tested for antibodies to Borrelia burgdorferi. Antibodies were
detected by enzyme-linked immunosorbent assay in 6 individuals (3%; 95% CI
: 1-5%), who were middle-aged, asymptomatic anti-nuclear and anti-smooth mu
scle antibody negative. Our data show that there is low seroprevalence rate
of antibodies to B. burgdorferi in an unselected south Estonian population
.