Detection of ferritin in human serum with a MAP-H2O2-HRP voltammetric enzyme-linked immunoassay system

A voltammetric enzyme-linked immunoassay based on new system of m-aminophen ol (MAP)-H2O2-horseradish peroxidase (HRP) has firstly been developed and u sed for the detection of HRP, labelled HRP and ferritin in human serum. HRP or labelled HRP catalyzes the oxidation reaction of MAP with H2O2, the pro duct of which produces a sensitive voltammetric peak at potential of - 0.46 V (vs. SCE) in Britton-Robinson (BR) buffer solution. By using this voltam metric peak, HRP can be measured with a detection limit of 9.5 x 10(-1) mU/ l and a linear range of 2.5-2.5 x 10(2) mU/l. The detection limit to ferrit in is 0.25 ng/ml and the linear range 0.25-320 ng/ml. The processes of the electro-reduction of the product of the enzyme-catalyzed reaction have been investigated in detail. (C) 1999 Elsevier Science B.V. All rights reserved .