Enantioselective oxidation of (RS)-2-phenyl-1-propanol to (S)-2-phenylpropanoic acid with Gluconobacter oxydans: simplex optimization of the biotransformation

Authors
Molinari, F Villa, R Aragozzini, F Leon, R Prazeres, DMF
Citation
F. Molinari et al., Enantioselective oxidation of (RS)-2-phenyl-1-propanol to (S)-2-phenylpropanoic acid with Gluconobacter oxydans: simplex optimization of the biotransformation, TETRAHEDR-A, 10(15), 1999, pp. 3003-3009
Citations number
11
Categorie Soggetti
Organic Chemistry/Polymer Science
Journal title
TETRAHEDRON-ASYMMETRY
ISSN journal
09574166 → ACNP
Volume
10
Issue
15
Year of publication
1999
Pages
3003 - 3009
Database
ISI
SICI code
0957-4166(19990730)10:15<3003:EOO(T(>2.0.ZU;2-H
Abstract
The microbial oxidation of racemic 2-phenyl-1-propanol by Gluconobacter oxy dans DSM 50049 was investigated. Whole bacterial cells were used to produce (S)-(+)-2-phenylpropanoic acid with high enantiomeric excess (E>200). A si mplex sequential method was employed as an experimental design to guide the optimization process. Temperature of 26-28 degrees C, pH 6.0-6.2, substrat e concentration of 20-25 mM and agitation of 150 rpm have been found the be st conditions to achieve the highest reaction rates and enantioselectivitie s. (C) 1999 Elsevier Science Ltd. All rights reserved.