P. Totte et al., Bovine CD4(+) T-cell lines reactive with soluble and membrane antigens of Cowdria ruminantium, VET IMMUNOL, 70(3-4), 1999, pp. 269-276
Cowdria-specific CD4(+) T-cell lines generated from immunised cattle respon
d to both soluble and membrane proteins of the agent, Furthermore, the line
s produced the Cowdria-inhibitory cytokine IFN-gamma in response to soluble
antigens fractionated by gel filtration and FPLC. Activity eluted as a sin
gle peak around fraction 15 for all T-cell lines tested. This fraction indu
ced the highest production of IFN-gamma by the lines and was shown by SDS-p
olyacrylamide gel electrophoresis and silver staining analysis to contain l
ess than 10 different bands ranging from 22 to 32 kDa. Given their high sen
sitivity and specificity, these short-term CD4(+) T-cell Lines will be valu
able tools for the identification of Cowdria antigens for incorporation in
a subunit vaccine. (C) 1999 Elsevier Science B,V, All rights reserved.