Structure-function analysis of the feline herpesvirus virulence factors gEand gI

The biosynthesis and function of the gE-gI complex of feline herpesvirus (F HV) was studied by heterologous expression of the cloned genes in the vacci nia virus-based vTF7-3 expression system and by analysis of FHV recombinant s. This work has led to the identification of domains in gI involved in com plex formation with gE, and to a model for the disulfide-bonded structure o f gI. The effects of mutations in gI on gE-gI-dependent cell-to-cell transm ission are discussed. (C) 1999 Elsevier Science B.V. All rights reserved.