Pullulan production from brewery wastes by Aureobasidium pullulans

The production of pullulan from brewery wastes by Aureobasidium pullulans i n shake flask culture was investigated. The maximum pullulan concentration (6.0 g/l) was obtained after 72 h of fermentation. The external addition of nutrients into the spent grain liquor improved significantly the productio n of pullulan. In this case, the highest values of pullulan concentration ( 11.0 +/- 0.5 g/l), pullulan yield (48.2 +/- 1.5%), and sugar utilization (9 9.0 +/- 0.5%) were obtained in the medium (pH 6.5-7.5) supplemented with K2 HPO4 0.5%, L-glutamic acid 1%, olive oil 2.5%, and Tween 800.5%.