Various techniques used to immobilize naringinase produced by Penicillium decombens PTCC 5248

Authors
Norouzian, D Hosseinzadeh, A Inanlou, DN Moazami, N
Citation
D. Norouzian et al., Various techniques used to immobilize naringinase produced by Penicillium decombens PTCC 5248, WORLD J MIC, 15(4), 1999, pp. 501-502
Citations number
7
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
ISSN journal
09593993 → ACNP
Volume
15
Issue
4
Year of publication
1999
Pages
501 - 502
Database
ISI
SICI code
0959-3993(199908)15:4<501:VTUTIN>2.0.ZU;2-B
Abstract
Naringinase produced by Penicillum decombens PTCC 5248 was immobilized by e ntrapping it in calcium alginate beads, hen egg white, gelatin open pore ma trix and covalently attaching it to activated seeds of Ocimum basilicum. Op timum pH and temperature were found to be 4, 4.5, 4.5, 3.5 and 65 degrees C , 60 degrees C respectively for enzyme immobilized by each technique mentio ned. Naringinase immobilized onto activated seeds of Ocimum basilicum was a ctive for 7 days.