1. 5'-Hydroxycotinine-N-oxide, 5-(3-pyridyl-N-oxide)-5-hydroxy-1-methyl-pyr
rolidone-2, was identified as a new in vivo metabolite of nicotine.
2. The new metabolite was isolated from the urine of rats treated with S-ni
cotine and characterized using chemical and spectrometric methods.
3. 5'-Hydroxycotinine-N-oxide was synthesized and characterized by MS and b
y infrared as well as H-1- and C-13-NMR spectroscopy.
4. Identity of the new metabolite with synthetic 5'-hydroxycotinine-N-oxide
was demonstrated by comparing the MS and H-1-NMR spectroscopy data as well
as by cochromatography of a spiked urine sample.