Analysis of micronuclei induced under hyperthermic conditions in human lymphocyte culture by fluorescence in situ hybridization (FISH) and spectral karyotyping (SKY) methods
N. Komae et al., Analysis of micronuclei induced under hyperthermic conditions in human lymphocyte culture by fluorescence in situ hybridization (FISH) and spectral karyotyping (SKY) methods, YAKUGAKU ZA, 119(10), 1999, pp. 763-772
Citations number
37
Categorie Soggetti
Pharmacology & Toxicology
Journal title
YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN
The spectral karyotyping (SKY) method is a novel molecular cytogenetic tech
nique which simultaneously discerns entire chromosomes. In order to elucida
te the origins of micronuclei induced under hyperthermic conditions in huma
n lymphocyte culture, peripheral blood cells were cultured at 40 degrees C
or 42 degrees C for 3-24h, using the cytokinesis-block method with cytochal
asin B. The induced micronuclei were identified by the fluorescence in situ
hybridization (FISH) and SKY methods. At 42 degrees C for more than 6 h, t
he frequency of occurrence of micronuclei in binucleated cells rose with in
creasing incubation time. By the FISH method, 83.3% of micronuclei induced
in 24 h culture at 42 degrees C were shown to be positive for the human cen
tromeric probes. By the SKY method, each micronucleus induced under the hyp
erthermic conditions was identified unequivocally and shown to contain a sp
ecific chromosome. These results suggest that the micronuclei induced under
the hyperthermic conditions in human lymphocyte culture contain chromosome
s which do not migrate to the poles at the anaphase of the cell cycle becau
se of the breakdown of the spindle apparatus.