Study of the reaction between nucleic acids and Tb-BPMPHD-CTMAB complex and its analytical application

The fluorescence quenching of the Tb-BPMPHD-CTMAB by nucleic acids is repor ted. The maximum fluorescence quenching was obtained at pH5.5, with maximum excitation and emission wavelengths at 300 and 543nm, respectively. Under optimal conditions, the differential value of fluorescence intensity betwee n the absence and presence of nucleic acids was proportional to the concent ration of nucleic acids over the range of 4.0x10(-8) ~ 1.0x10(-5) g/ml for fsDNA and 2.5x10(-8) ~ 6.15x10(-6) g/ml for yRNA, respectively. The corresp onding detection limits are 9.0ng/ml for fsDNA and 5.0ng/ml for yRNA. The m ethod was applied to the determination of DNA (or RNA) in synthetic samples . The results obtained were satisfactory.